DETERMINATION OF IRIDOID, ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUNDS BY HPLC METHOD IN PLANTS GENTIANA CRUCIATA L. FROM DIFFERENT GROWTH PLACE

Abstract

Background. The ecological and geographical conditions of the habitat may be responsible for the synthesis and accumulation of various bioactive compounds and, therefore, for biological effects, including the antioxidant properties of medicinal plants. Understanding the impact of environmental factors on bioactive compounds in medicinal plants have great importance for choosing a place for growing and harvesting plants with a high content of bioactive compounds, as well as providing the highest quality raw materials in the pharmaceutical industry.
 Objective. To study the accumulation of iridoids, phenolic compounds and antioxidant activity in G. cruciata plant samples, depending on the growing conditions.
 Results. The regions of the Republic of Tatarstan with the best quality of medicinal raw materials, as well as factors contributing to the accumulation of various groups of biologically active substances in G. cruciate plants, were identified. Humidity and soil type played a decisive role in the accumulation of iridoids; the synthesis of phenolic compounds was more influenced by humidity than the type of soil. However, since the change in antioxidant activity did not correspond to concentration of phenolic compounds, this may be due to the presence of iridoids, which are considered to be the main substances of the plant of the genus Grecian. Their antioxidant activity has been shown in previous studies. In our studies, soil properties seem to have great influence on the production of studied compounds.
 Conclusions. Almetevsky and Laishevsky districts can be chosen as the optimal place for growing and collecting medicinal plants G. cruciata L.