Abstract
Development of a method for the determination of insulin based on capillary electrophoresis with laser-induced native fluorescence detection is described. Under optimal conditions, insulin as low as 73 amol can be detected with a good signal-to-noise ratio (S/N = 10 peak-to-peak). Application of this method for the determination of insulin content in single cells from the insulin-secreting cell lines RINm5F and beta TC3 is demonstrated. Non-bonded poly(ethylene oxide)-coated and bare capillaries are evaluated for this purpose, with the latter found to be more suitable for single-cell analysis.
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