Abstract
The presence of nonionic and cationic detergents or protein interfered with the Fiske and Subba Row method for determination of inorganic phosphate by causing precipitate formation. The addition of 1 ml of 20% sodium dodecyl sulphate to the sample prevented precipitation by all the compounds tested without affecting the colour development. Applications include the simplified assay of phosphatases including those released from membranes by detergents.
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