Determination of inorganic mercury and total mercury in biological and environmental samples by flow injection-cold vapor-atomic absorption spectrometry using sodium borohydride as the sole reducing agent

Abstract

A simple, fast, precise and accurate method to determine inorganic mercury and total mercury in biological and environmental samples was developed. The optimized flow-injection mercury system permitted the separate determination of inorganic mercury and total mercury using sodium borohydride as reducing agent. Inorganic mercury was selectively determined after reduction with 10 −4% w/v sodium borohydride, while total mercury was determined after reduction with 0.75% w/v sodium borohydride. The calibration graphs were linear up to 30 ng ml −1. The detection limits of the method based on three times the standard deviation of the blank were 24 and 3.9 ng l −1 for total mercury and inorganic mercury determination, respectively. The relative standard deviation was less than 1.5% for a 10 ng ml −1 mercury standard. As a means of checking method performance, deionized water and pond water samples were spiked with methylmercury and inorganic mercury; quantitative recovery for total mercury and inorganic mercury was obtained. The accuracy of the method was verified by analyzing alkaline and acid extracts of five biological and sediment reference materials. Microwave-assisted extraction procedures resulted in higher concentrations of recovered mercury species, lower matrix interference with mercury determination and less time involved in sample treatment than conventional extraction procedures. The standard addition method was only needed for calibration when biological samples were analyzed. The detection limits were in the range of 1.2–19 and 6.6–18 ng g −1 in biological and sediment samples for inorganic mercury and total mercury determination, respectively.