Abstract
Liquid chromatography and pulsed amperometric detection with a gold working electrode have been used for the determination of cellobiose and rhamnose in urine samples. A conventional sugar-separation column was used with an acidic mobile phase, and a potassium hydroxide solution was added post-column. Under optimal conditions detection limits of 10 ng were obtained. The method could be used for the assessment of the human intestinal permeability by the determination of the urinary excretion of inert sugars. Limits of determination in urine were 5 mg l−1 for cellobiose and 15 mg l−1 for rhamnose. Good agreement was found with the results of alternative methods.
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