Abstract
On-line reversed-phase capillary liquid chromatography/tandem mass spectrometry with electrospray ionization was used for the detection of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) adducts of DNA and compared to analysis by 32P-postlabeling. Structural information was obtained on low nanogram levels of a synthetic N-(deoxyguanosin-8-yl)-PhIP (dG-C8-PhIP) standard using collision-induced dissociation, and low picogram level detection was achieved for the targeted dG-C8-PhIP adduct using the selective multiple reaction monitoring scanning mode. The method was applied to the analysis of an in vitro reaction mixture identifying the dG-C8-PhIP adduct and providing evidence for the presence of two additional PhIP-modified deoxyguanosine adducts. The results were in qualitative agreement with those obtained by the 32P-postlabeling method. In addition, the mass spectrometric results revealed the occurrence of an unexpected product in the in vitro reaction mixture presumably resulting from cleavage through the guanine base.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
