Abstract
Abstract A liquid chromatography method for the detection and quantification of 5-hydroxymethyl-2-furaldehyde (HMF) in baby cereals is described. HMF was obtained by centrifugation and the supernatant was clarified by the addition of Carrez solution. The mixture was centrifuged for 5 min at 5,000 rpm. The samples were analysed on a reverse-phase C18 column and ultraviolet detector (284 nm) in isocratic elution with 95:5 water-acetonitrile. Average recovery of HMF by the standard addition method was 96.9%. The variation coefficients for three levels of concentration, (3.24, 22.82 and 65.50 ppm) were 2.40. 2.14 and 1.09% respectively. Limit of detection was ≥0.09 ppm. This method was applied by determination of HMF in commercial baby cereals.
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