Abstract
The development and characterisation of a new organic phase enzyme electrode (OPEE) for hydroperoxides is described. The biosensor was obtained by combining an oxygen gas diffusion amperometric electrode and two immobilised enzymes (peroxidase and tyrosinase) working in parallel and competing for the same substrate (catechol). LOD was about 0.1×10−3moll−1. The biosensor was applied for the determination, using decane as solvent, of the “pool” of hydroperoxides released during the heating (to about 130–140°C) of extravirgin olive oil, or used to determine the hydrogen peroxide content of lipophilic cosmetic products, working in a dioxane–water mixture.
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