Determination of human serum immunoglobulin G using flow injection analysis with rate turbidimetric detection.

Abstract

An immunological reaction between human serum immunoglobulin G (IgG) and goat anti-human IgG was investigated using a fully automated stop-flow merging zones flow injection analysis manifold. Turbidimetric detection at 340 nm was used to monitor the rate of reaction. A sampling rate of 40 samples per hour and a precision of 2.0–6.8% RSD (relative standard deviation) were obtained for a range of human serum standards. Serum samples and a human reference serum were analysed and their IgG concentrations interpolated from a second-order fit of the calibration data. The consumption of expensive antiserum was less than 1 µl per assay.