Abstract
Luminescent silicon dioxide nano-particles with size of 20 nm, which containing dibromofluorescein (D) were synthesized by sol–gel method (symbolized by D–SiO 2).The particles can emit intense and stable room temperature phosphorescence signal on polyamide membrane when Pb(Ac) 2 was used as a heavy atom perturber. The λ exmax/ λ emmax was 457/622 nm. Our research indicated that the specific immune reaction between goat-anti-human IgG antibody labeled with D–SiO 2 and human IgG could be carried out on polyamide membrane quantitatively, and the phosphorescence intensity of the particle was enhanced after the immunoreactions. Thus a new method of solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA) for the determination of human IgG was established basing on antibody labeled with the D–SiO 2 nanoparticles. The linear range of this method was 0.0624–20.0 pg human IgG spot −1 (corresponding concentration: 0.156–50.0 ng ml −1, the sample volume: 0.40 μl spot −1) with a limit of detection (LD) as 0.018 pg spot −1, and the regression equation of working curve was Δ I p = 7.201 m IgG (pg spot −1) + 82.57. Samples containing 0.156 and 50.0 ng ml −1 of IgG were measured repeatedly for 11 times and R.S.D.s were 4.1 and 3.4%, respectively. Results showed that this method had the merits as sensitive, accurate and precise.
Published Version
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