Determination of Histamine and Tyramine in Canned Fish Samples by Headspace Solid-Phase Microextraction Based on a Nanostructured Polypyrrole Fiber Followed by Ion Mobility Spectrometry

Abstract

This paper describes the development of a simple, rapid, and selective method based on headspace solid-phase microextraction (HS-SPME) combined with ion mobility spectrometry (IMS) for the simultaneous determination of histamine (HIS) and tyramine (TYR) in the canned fish samples. The spectra interferences were eliminated by using a new alternate reagent gas and resulted in an increased sensitivity and selectivity of IMS technique. A dodecylbenzenesulfonate-doped polypyrrole coating was used as a fiber for HS-SPME method. The calibration curves were linear in the range of 30–300 ng g−1 (R2 > 0.994). Limits of detection for HIS and TYR were 3 and 4 ng g−1, respectively. The proposed method was successfully applied to determine HIS and TYR in different canned fish samples without derivatization steps. Method validation was conducted by comparing our results with those obtained through gas chromatography-mass spectrometry method.