Abstract
A method has been developed for the determination of lipoprotein lipase activity (LLA) in post-heparin plasma, utilising a commercially available fat emulsion, Intra-lipid (R), as substrate. Optimal conditions for the enzyme reaction with regard to substrate concentration, pH, temperature and albumin concentration have been worked out. The enzyme kinetics for Intralipid (R) and chylomicrons were very similar whereas other substrates, as e.g. Ediol (R), had quite different properties in that respect. Sodium chloride, 1 M, inhibited the lipolysis in post-heparin plasma over 90%. Other inhibitors were also studied. The LLA in plasma after intravenous administration of heparin reaches a peak value and then decreases exponentially.
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