Abstract
Fluorescence of 8-anilinonaphthalene-1-sulfonate (ANS) was found to be enhanced by heparin-protamine complex. Heparin was found to bind with protamine electrostatically and the highest fluorescence was observed when the concentrations of sulfate groups of heparin and arginine moieties of protamine were equal. The increase in the fluorescence intensity of ANS was assumed to be caused by its interaction with the hydrophobic regions induced in the protamine molecule by the interaction with heparin. The phenomenon was applicable to the determination of heparin.
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