Abstract
Background: Highly selective and sensitive analysis of hydrogen peroxide (H2O2) has attracted considerable interest in the fields of clinical diagnostics, food industry, and environmental analysis. Objectives: In the present study, an efficient electrochemical sensor, based on V2O5/VO2 nanostructures, was introduced for measuring hydrogen peroxide (H2O2) in human serum samples. Methods: The characterization of the prepared V2O5/VO2 nanostructures was investigated by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). A carbon paste electrode (CPE) modified with V2O5/VO2 was applied for the electrochemical detection of H2O2. Results: The prepared sensor depicted a good linear range from 8 to 215 μM and a low detection limit of 5 μM. Moreover, the modified electrode showed notable anti-interference property and high sensitivity toward H2O2 detection. The suggested method was also successfully applied for the determination of H2O2 in human serum samples. Conclusions: The V2O5/VO2 embedded CPE offers good simplicity, sensitivity, and selectivity toward H2O2 determination. In addition, the suggested assay revealed good reproducibility and anti-interference property in the measuring of H2O2.
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