Abstract
A method for determining glycerol in wines by high-performance liquid chromatography has been developed using three 250 × 8 mm columns based on a sulphonated styrene-divinyl benzene polymer (SHODEX S-801/S,S-802/S) in series. This polymer combines cation-exchange mechanisms with molecular exclusion. The columns were thermostated at 75°C using water as the mobile phase. A refraction index detector was used and the samples were directly injected. The method is reproducible (coefficient of variation above 1%) and accurate. Linear regression analysis and Student's t-test for comparison of the mean show that the results are comparable with those obtained by an enymatic method (differences less than 4%).
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