Abstract
A method has been developed to easily separate and quantify glucosinolates in mustard and winter cress plants using reversed-phase HPLC with volatile ion-pairing reagents to modify the mobile phase and enhance retention and selectivity. Volatile buffers are required for HPLC-electrospray mass spectrometry. Confirmation of identity was accomplished using both negative ion electrospray mass spectrometry and photodiode-array detection. This method provides a way to determine glucosinolates in Brassica and cruciferous vegetables, most of which are commercially unavailable. Glucosinolates were determined in various parts and sections of yellow and brown domestic mustards and winter cress (wild mustard). Results varied from 0.2 mg/g glucosinolate content in the roots of the plants to 26 mg/g in the seeds.
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