Abstract
Abstract Microfluidic enzyme-based reactor could be used to detect biomolecules with high sensitivity and selectivity. Based on the electrophoretic-mediated microanalysis (EMMA) method, a novel free enzyme-based microchip was developed to detect glucose. Online catalysis reaction of glucose by glucose oxidase was carried out on the chip. The product H 2 O 2 was detected using amperometric method. The working electrode, single carbon fibre cylindrical electrode, was held at a constant potential of +1.4 V versus Ag/AgCl electrode. The results indicated that the peak current had a good linear relationship with glucose concentration in the range of 0.1–20 mM ( R = 0.997) with good reproducibility (RSD = 2.02%, n = 10). The detection limit of glucose was 19.8 μM ( S/N = 3). This free enzyme-based reactor was easy to fabricate and operate, which showed great potential application in bioanalysis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
