Abstract
The paper establishes a new HPLC method for measurement of the total content of gluconic acid (GA) in wine using a pulsed amperometric detector equipped with a gold electrode cell and an anion exchange column. A NaOH solution was used at a flow rate of 1.5 ml·min -1 as a mobile phase under isocratic conditions. Analysis took 30 min. The linearity of the method was shown up to 500 mg·l -1 , with a LOQ of 2.0 mg·l -1 (10σ). Precision (as RSD%) was under 2.5 % in the usual range of GA concentration in natural wines. Accuracy was checked in comparison with the usual enzymatic method.
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