Abstract
Abstract The 3 major components of gentamicin sulfate (C1a, C2, and C1) were separated by a liquid chromatographic method using a reverse phase C18 bonded silica gel column and an aqueous ion pair solvent system consisting of 0.015M pentanesulfonic acid sodium salt, 0.2M sodium sulfate, and 0.1% acetic acid. Individual C components as well as several unknown minor constituents were detected by an electrochemical flow cell with a glassy carbon working electrode set at a potential of +1.3 ± 0.1 V vs Ag/Agd. The analytical response was linear over a concentration range of 16-30 μg. A plot of peak areas vs concentration of C fractions yielded a correlation coefficient of 0.999 or better for the Cla, C2, and C1 components of gentamicin sulfate.
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