Abstract
Some Trichoderma isolates were collected from different locations of Bangladesh for evaluating their bioefficiency by determining their genetic variations. PCR-based Random Amplified Polymorphic DNA (RAPD) Marker employing 3 decamer primers produced 29 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high level of genetic diversity among the isolates. The Unweighted Pair Group Method of Arithmetic Means (UPGMA) dendrogram constructed from Nei’s (1972) genetic distance produced 2 main clusters (16 isolates in cluster 1 and 19 isolates in cluster 2). The result indicating their genetic diversity has opened new possibility of using the most efficient and more isolates of Trichoderma in the preparation of effective biopesticide. Keywords: Genetic diversity; Trichoderma; RAPD DOI: http://dx.doi.org/10.3329/jbau.v9i1.8738 JBAU 2011; 9(1): 9-20
Highlights
Trichoderma is a soil fungi which reproduces asexually which is frequently isolated and most prevalently culturable
Primer selection and Random Amplified Polymorphic DNA (RAPD) pattern: Six primers were initially screened for their ability to produce polymorphic patterns among 35 isolates of Trichoderma spp. and three primers viz., OPB-09, 0PB-10, and OPC-01 which showed comparatively maximum number of high resolution bands and distinct polymorphic amplified products were selected
It may be concluded that T24, T27, T28 and T29 were originated from same parent or closely related parent
Summary
Trichoderma is a soil fungi which reproduces asexually which is frequently isolated and most prevalently culturable. It is widely distributed in plant material, decaying vegetation, wood and other diverse habitats (Ranasingh, 2006). Trichoderma spp. are facultative anaerobes, grow saprophytically or as parasites on other Trichoderma and are able to grow in soils having a pH range of 2.5 - 9.5, most of them prefer moderately acidic environment (Grondona, 1997). Most of the Trichoderma strains produce only asexual spores. A few strains have sexual stage which are not considered for biocontrol purposes. Morphological features were considered to determine different taxonomical aspects with the help of the asexual sporulation apparatus, but molecular approaches are being used
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