Abstract
Background: In Turkey, especially cutenous leishmaniasis is endemic and Sanliurfa has the highest endemicity rating in Turkey in terms of CL; the yearly case number is around 2000. According to the data of the Turkish Ministry of Health, 23,219 of 46,003 CL cases (50.47%) reported in Turkey between 1990-2010 were from Sanliurfa. In Sanliurfa/Turkey, the only causative agent identified to date has been Leishmania tropica which is known to be transmitted antroponotically. However, it is thought that L.tropica species that circulate around the region are showing intra-species differences. It has been seen that there are differences in lesion characteristics, process and response to the drug between people who have been infected by L.tropica. After this pre-study, the importance of researching the genetic differences in population of L.tropica circulating in Sanliurfa was observed. Methods & Materials: To obtain isolates, clinical specimens taken from indigenous and Syrian CL patients are going to be inoculated in NNN. Then, diagnosis and species identification have done by ITS1-PZR-RFLP. PCR have been performed on 50 samples that have been diagnosed with L.tropica with primers determined for 12 microsatellite marker. The products have been carried out on PAGE (Poliakrilamid gel elektrophorez). Tape lengths and differences between them on the imaging device have been determined. The data which have been obtained by MLMT, have been analyzed with STRUCTURE software for the purpose of detecting and resolving the parasite’s population structure and isolates’ genetic relationships and proximity. Results: STRUCTURE analysis identified only one main populations among all 50 isolates (K:1). Syrian isolates were genetically identical to S,anliurfa strains. It was found that all of the L.tropica strains obtained belong to the same population. In the analyzes made, L. tropica samples started to show differentiation at the locus level so as not to change the number of populations. This difference is statistically insignificant as suggested by STRUCTURE analysis. Conclusion: This repetition is currently not creating a new population size. it is thought that this may change in the future. For this reason, it was thought that it would be beneficial to study small differences in locus level with advanced study methods.
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