Determination of Genetic Diversity inSclerotium rolfsiiandSclerotium delphiniiby using RAPD and ISSR Markers

Abstract

The classification and variability among Sclerotium species was studied by using ISSR and RAPD markers. Based on the amplification pattern and reproducibility of eleven RAPD premiers and seven ISSR primers, the delineating variability among the 13 different crops isolates of Sclerotium rolfsii and 4 isolates of Sclerotium delphinii was analyzed. The primer OPB-16 was found to be the best primer for determination of variability among Sclerotium spp. This primer generated at least 17 bands of 230 -1554 base pairs size. Similar results were obtained with IS-8 primer in ISSR marker amplification studies. Though all the isolates of Sclerotium spp. were genetically variable, they share some common bands indicating phylogenetic relationship among them. The ability of primers to highlight the genetic polymorphism between the isolates varied greatly and on the basis of pooled RAPD and ISSR marker analysis these isolates were classified into five broad groups. RAPD-PCR and ISSR molecular marker analysis were found to be efficient and informative technique for differentiating the genetic variability among Sclerotium species.