Determination of Genetic Diversity and Screening of BCMV and BCMNV Resistance in Some Bean Genotypes Using Molecular Markers

Abstract

Fresh bean (Phaseolus vulgaris L.) is an important legume crop in Turkey used as a major source of protein in the human diet. The total fresh bean production of Turkey is estimated at 651,094 tons. Bean production shows a decreasing factor due to various abiotic and biotic factors in Turkey. Among the viruses that infect beans, Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) are the most widespread destructive agents. The main objective of this study was to carry out a diagnostic survey for Bean common mosaic disease (BCMD) in beans, characterize its causal agent, and evaluate host resistance to BCMV and BCMNV. In addition to this, genotypes were tested for the levels of genetic diversity by 58 SSR markers. A total of 123 alleles with a PIC value of ≥0.2 were obtained from 75 bean genotypes. The unweighted NJ dendrogram was created using the scoring data, which demonstrated the molecular genetic relationships among the bean genotypes. SCAR (Sequence Characterized Amplified Region) markers were used to determine the resistant genotypes to BCMV and BCMNV. According to the result, 21 out of 75 genotypes have resistance alleles to both pathogens.