Abstract
Gamma aminobutyric acid (GABA) has great physiological functions, mainly as a major inhibitory neurotransmitter in the brain, which makes it important for the development of functional foods. This study detected GABA in grains and cruciferous vegetable seeds by using HPLC after pre-column on-line derivatization with diode array detection (DAD) and fluorescence detection (FLD). The limit of quantitation was 2.94 and 2.86 µg/mL with DAD and FLD, respectively. GABA recoveries ranged from 98.8 to 111.2% on both detectors. Intra and inter-day precision showed relative standard deviations, generally, less than 10% for both DAD and FLD. GABA was determined in different grains (flaxseeds, white quinoa seeds, and buckwheat) and cruciferous vegetable seeds (broccoli, kale, daikon radish, mustard, cabbage, and brussels sprouts). Organic broccoli seeds contained the highest amount and mustard seeds the least amount of GABA in the Brassica family with none being detected in organic white quinoa and flaxseeds. A statistically significant difference (p < 0.05) exists between the various lots of the broccoli seeds. GABA is important as a natural source in functional foods.
Highlights
Gamma aminobutyric acid (GABA) is a four-carbon non-proteinogenic amino acid and a major inhibitory neurotransmitter in the brain
GABA was determined in different grains and cruciferous vegetable seeds
To optimize the extraction of GABA, water and sonication were used for the analyses of grains and cruciferous vegetable seeds
Summary
Gamma aminobutyric acid (GABA) is a four-carbon non-proteinogenic amino acid and a major inhibitory neurotransmitter in the brain. It has several physiological roles, mainly in the human central nervous system (Watanbe, Maemura, Kanbara, Tamayama & Hayasaki, 2002). It is believed to help prevent diabetes (Taneera et al, 2012; Braun et al, 2010). GABA can delay and/or prevent the spread of cancer cells and stimulate apoptosis in cancer cells. It is shown to suppress tumors as well (Diana, Quílez, & Rafecas, 2014)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
