Abstract
Galacturonic acid (GalA) in pomelo pectin was converted to galactose (Gal) by saponification and reduction reaction of carboxy group. The characterization of the product was investigated by 1H NMR spectroscopy. The reduced pectin was then hydrolyzed with trifluoroacetic acid (TFA) and analyzed by high-performance anion-exchange chromatography (HPAEC) with fluorescence detector. The content of GalA was calculated from an increased Gal after reaction. The GalA content obtained from this method was higher than that obtained from HPAEC-pulsed amperometric detection (PAD), which affords to analyze GalA directly after hydrolysis. The established method was validated and the results showed good linearity, high precision and high sensitivity. This method could be further applied for the analysis of GalA content in pomelo pectin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
