Abstract
γ-Aminobutyric acid (GABA)-induced Cl − fluxes in cultured rat cerebellar granule cells were measured using the chloride-sensitive fluorescent probe SPQ (6-methoxy- N-(3-sulphopropyl)quinolinium) incorporated into the cells. The fluorescence of SPQ is quenched by Cl − ions. GABA and pentobarbitone increased the fluorescence of the probe when the Cl − gradient was directed outward by bathing cells, grown in the presence of GABA, in a low Cl − medium. Picrotoxin and bicuculline inhibited the response to GABA. The results suggest that SPQ is a suitable probe for measuring GABA-induced Cl − fluxes in living cells.
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