Abstract
The effect of dilution of serum on the concentration of free thyroxine was evaluated in an effort to determine the relationship between values previously obtained in a standard dialysis system employing diluted serum (1:150) and the concentration of free thyroxine in undiluted system. A theoretical analysis of thyroxine-binding by serum proteins in terms of a multiple equilibria system suggested that the free thyroxine concentration should be relatively independent of the concentration of serum proteins in the range of dilution considered. In order to test this prediction, experimental techniques were devised to measure free thyroxine in dialysis systems containing higher concentrations of protein. For this purpose, tracer 131I-thyroxine and 125I-iodide were used simultaneously. The results of these studies were in general accord with the theoretical formulation. The concentration of free thyroxine was also measured in the ultrafiltrate of 7 sera. Values were in the same order of magnitude as those obtain...
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