Determination of free- and protein primary amino acids in biological materials by high-performance liquid chromatography and photodiode array detection

M Czuderna,J Kowalczyk,I Wąsowska,K Niedźwiedzka

doi:10.22358/jafs/67798/2002

Abstract

Determination of free- and protein primary amino acids in biological materials by high-performance liquid chromatography and photodiode array detection

Highlights

The response o f animals to feed protein (PN) and non-protein nitrogen sources (NPN) basically depends on the amount and nature o f feed protein and amino acids absorbed from the small intestine
Many high performance liquid chromatography (HPLC) methods and gas chromatography techniques (GC) for quantification o f amino acids have been published in recent years (Sarwar and Botting, 1993; Cohen and Michaud, 1998; Czauderna and K o walczyk, 1998; Peter et al, 1998; Albin et al, 2000; Polak and Golkiewicz, 2000; Kutlan and Molnar-Perl, 2001; Molnar-Perl, 2001).The major shortcomings o f automatic amino acid analyzers for amino acids observed are long analysis time and inadequate detection limits, dedication of the analyzers to only amino acid analysis, and high cost o f the instruments
The aim o f this work was to extend our earlier investigations (Czauderna and Kowalczyk, 1998, 1999) in order to determine the possibility o f free- and protein amino acid assays in biological materials

Summary

Introduction

The response o f animals to feed protein (PN) and non-protein nitrogen sources (NPN) basically depends on the amount and nature o f feed protein and amino acids absorbed from the small intestine. In recent years much interest has focused on covering amino acid requirements o f animals for growth, development and productivity (Batterham, 1992; Lewis and Bayley, 1995; Zebrowska and Buraczewska, 1998; Ravindran and Bryden, 1999). Many high performance liquid chromatography (HPLC) methods and gas chromatography techniques (GC) for quantification o f amino acids have been published in recent years (Sarwar and Botting, 1993; Cohen and Michaud, 1998; Czauderna and K o walczyk, 1998; Peter et al, 1998; Albin et al, 2000; Polak and Golkiewicz, 2000; Kutlan and Molnar-Perl, 2001; Molnar-Perl, 2001).The major shortcomings o f automatic amino acid analyzers for amino acids observed are long analysis time and inadequate detection limits, dedication of the analyzers to only amino acid analysis, and high cost o f the instruments.

Methods

Results

Conclusion