Abstract
Unconjugated triclosan in blood can be determined by extraction with hexane, followed by its conversion to an acetyl derivative and analysis by electron capture gas liquid chromatography. The conjugated triclosan can be analyzed by an identical procedure, after the complexes have been hydrolyzed with hydrochloric acid. The method was validated by recovery studies using spiking and radioactive techniques and can be used for the determination of triclosan in blood in the concentration range of 1 ppb (ng/ml) to 100 ppm (µg/ml). The precision and accuracy of the method are discussed, and examples of its application are presented.
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