Abstract

Free and total Mn(II) content of hepatocytes from fed and fasted rats has been determined by EPR. The difference spectra of cumulative scans of cell samples and the mother medium allows a sensitivity of 0.1 PM free Mn(II). The free and total Mn(II) content was 0.71 2 0.10 and 34.4 f 4.4 nmol/ml of cell H20 in hepatocytes from fed animals and was 0.25 -+, 0.15 and 35.9 +. 7.6 nmol/ml of cell HzO, respectively, in hepatocytes from fasted animals. These results demonstrate a 2.8-fold decrease in free Mn(I1) with no change in the total cell Mn(II) in response to fasting.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.