Determination of Folic Acid by CE in Various Cultivated Variety of Lentils

Abstract

A simple extraction and determination method for folic acid (FA) in lentil samples was developed employing capillary electrophoresis. The analysis was performed in a 75 μm ID fused silica capillary using a running buffer of 10 mM sodium borate (10%, v/v, methanol, pH 9) at +18 kV and a detection wavelength at 200 nm. Methylparaben was used as the internal standard. FA signal response was linear in the range between 1.2 × 10−5 and 4.8 × 10−5 M. Limit of detection (inter-day) was determined at 6.12 × 10−7 M (3.3 σs−1). The amount of FA found in green, red and mignon lentils was found to range between 0.408 and 0.742 mg g−1.