Abstract
A multi-residue method developed for the analysis of fluoroquinolone in Penaeus japonicus muscular tissue is presented. The method is based on microwave-assisted extraction (MAE) of Penaeus japonicus muscular tissue using acetonitrile as extractant and analysis of extracts by ion-pair high performance liquid chromatography (HPLC) and fluorescence detection. MAE operational parameters, the solvent type and volume, extraction temperature, and time were optimized in detail with respect to extraction efficiency of the target compounds from Penaeus japonicus muscular tissue. The recoveries of the method at three different spiked levels were assessed by analyzing spiked meat samples and were found to be in the range from 78.40 to 96.62% with good precision (<10%).
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