Abstract
A simple liquid chromatographic (LC) method was developed for the determination of fluoroquinolones (ciprofloxacin, difloxacin, enrofloxacin and sarafloxacin) in animal tissues. Isolation of fluoroquinolones from biological matrices was performed with 5% trichloroacetic acid-acetonitrile (7:3) solution. For clean-up, solid-phase extraction with an SDBI (styrene–divinylobenzene) cartridge was used. LC analyses were performed with analytical column (LiChrospher 100 RP-8 5 µm) and mobile phase (0.025 M O-phosphoric acid–acetonitrile 70:30, v/v) in ion-pair mode. The whole procedure was validated in intra- and inter-assay reproducibility and accuracy determination by simultaneously assaying of muscle, liver and kidney samples supplemented with fluoroquinolones at the level of 30 and 60 ng/g, respectively. The statistical evaluation demonstrates high absolute recovery (>80%) and low coefficient of variation (<10%) for all analysed samples. The detection limits for fluoroquinolones were 5 ng/g in muscle, liver and kidney samples. Copyright © 1999 John Wiley & Sons, Ltd.
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