Abstract
This work describes the development of the analytical methodology for determining fipronil residues in honey and pollen samples by gas chromatography with two detecting systems: electron-capture detection (GC-ECD) and mass spectrometry (GC-MS). Fipronil was extracted from honey samples by solid-phase extraction, using alumina as adsorbent and hexane-ethyl acetate (1:1, v/v) as eluting solvent, and from pollen samples by matrix solid-phase dispersion with C18 and acetonitrile, assisted by sonication. The developed methods gave recovery results >90% with relative standard deviations
Highlights
This work describes the development of the analytical methodology for determining fipronil residues in honey and pollen samples by gas chromatography with two detecting systems: electron-capture detection (GC-electron-capture detectors (ECD)) and mass spectrometry (GC-MS)
Los límites de detección obtenidos con GC-ECD fueron 1 μg kg-1 para el polen y 0,5 μg kg-1 para la miel y los valores correspondientes para GC-MS fueron 0,2 y 0,1 μg kg-1, respectivamente
The aim of this study was to develop the analytical methodology for the determination of fipronil in honey and pollen samples, based on solid-phase extraction (SPE) and Matrix solid-phase dispersion (MSPD), respectively
Summary
This work describes the development of the analytical methodology for determining fipronil residues in honey and pollen samples by gas chromatography with two detecting systems: electron-capture detection (GC-ECD) and mass spectrometry (GC-MS). The limits of detection obtained with GC-ECD were 1 μg kg-1 for pollen and 0.5 μg kg-1 for honey and the corresponding values for GC-MS were 0.2 and 0.1 μg kg-1, respectively These methods were applied to the determination of fipronil levels in various samples of honey and pollen commercialised in Spain and no residues of this compound were detected. Matrix solid-phase dispersion (MSPD) is an extraction technique that has been used in the determination of fipronil in honeybees (Morzycka, 2002) These techniques, SPE and MSPD, were firstly applied by our laboratory for the extraction of fipronil from honey and pollen samples, respectively (Sánchez-Brunete et al, 2005a)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
