Abstract
A sensitive liquid Chromatographic method for the analysis of the major component of fetal hemoglobin (HbF 0) in blood using high-resolution strong cation exchange column is described. The imprecision of the method was assessed in 20 repeated assays of blood samples containing 0.67%, 0.97%, 2.2% and 3.3% of HbF 0. Within-assay CVs were 5.0%, 2.8%, 1.8% and 1.5% and between-assay CVs 5.8%, 3.7%, 1.9% and 2.7%, respectively. The HbF 0 concentration was stable in EDTA blood for at least 20 days at +8°C and 3 days at +22°C. Blood HbF 0 levels (mean ± S.D. were 0.41 ± 0.20% for 21 healthy males, and 0.48 ± 0.19% for 22 healthy non-pregnant females. The method is simple and reproducible, and allows the sensitive determination of blood HbF 0.
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