Determination of fenfluramine based on potassium permanganate–calcein chemiluminescence system

Abstract

Calcein was found to be able to use as chemiluminescence reagent and post-chemiluminescence was observed when fenfluramine was injected into the mixture after the CL reaction of calcein–potassium permanganate. Based on this phenomenon, a flow injection CL method was established for the determination of fenfluramine. The possible CL mechanism was proposed. The CL intensity was correlated linearly with the concentration of fenfluramine over the range of 1.0 × 10 −7 to 6.0 × 10 −6 g mL −1 and the detection limit was 6 × 10 −8 g mL −1. The relative standard deviation was 2.2% for 5.0 × 10 −7 g mL −1 fenfluramine ( n = 11). This method was applied to the determination of fenfluramine in weight-reducing tonic successfully.