Abstract
We present the potential use of two kinds of PCR-based markers, RAPDs (Random Amplified Polymorphic DNA) and SPARs (Single Primer Amplification Reactions), as tools for hybrid seed purity determination. Five F1 pepper hybrids (Capsicum annuum L.) and their parents were analyzed with 100 10-mer primers and 10 nucleotide repeat primers. We found at least one useful marker for testing the purity of all hybrids studied. Despite their dominant inheritance these markers could be an efficient implement in the process of quality testing of hybrid seeds.
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