Abstract
The essential oils of the Osmanthus fragrans tea (OFT), Osmanthus fragrans (OF) and green tea (GT) had been extracted by steam distillation and analyzed by gas chromatography-mass spectrometry (GC-MS) with the help of heuristic evolving latent projections (HELP), an effective chemometric resolution method (CRM). The overlapping peak clusters were resolved into pure chromatograms and pure mass spectra with HELP. The qualitative analysis was performed by similarity searches in the National Institute of Standards and Technology (NIST) mass spectra database with the obtained pure mass spectrum of each component. Identification of some compounds was also assisted by comparison of temperature-programmed retention indices (PTRI) with authentic standards included in our own laboratory database under construction. The quantitative results were obtained by overall volume integration (OVI) method. A total of 67, 73 and 53 components in essential oils of the OFT, OF and GT were identified, accounting for 90.83%, 93.65% and 89.97% total contents of the essential oil of OFT, OF and GT, respectively.
Highlights
China is the homeland of tea, taking a leading position in the planting, producing and drinking of tea
We focus on using the gas chromatography-mass spectrometry (GC-MS) hyphenated technique to get two dimensional data and on resolving the overlapping peaks into pure spectra and chromatograms with chemometric resolution method (CRM)
The combination of IT and the heuristic evolving latent projections (HELP) method greatly improved the accuracy of identification of the chemical components in our essential oil samples
Summary
Chun-Di Hu 1,2, Yi-Zeng Liang 2,*, Fang-Qiu Guo 2 , Xiao-Ru Li 2 and Wei-Ping Wang 3. Research Center of Modernization of Chinese Herbal Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. Received: 11 February 2010; in revised form: 11 March 2010 / Accepted: 21 April 2010 /
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