Abstract
A simple, precise and sensitive reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitation of Escitalopram oxalate in pharmaceutical formulations. Chromatographic separation was achieved on a 250 × 4.6 mm, 5μ, C 18 column. The flow rate was 1ml/min and eluent was monitored by absorbance at 240 nm using a mixture of Acetonitrile and Buffer (pH 4.0) in the ratio of 25:75 (v/v). The retention times of Escitalopram oxalate was found to be 3.2 min. Calibration plots were linear in the concentration range of 2.5-80 μg mL -1 for Escitalopram oxalate. The total run time is 10 min. The proposed method was validated by testing its linearity, recovery, specificity, system suitability, precision (Interday and intraday), robustness and LOD/LOQ values and it was successfully employed for the determination of Escitalopram oxalate in pharmaceutical tablet formulations.
Highlights
Escitalopram oxalate (ESC) is the (Figure 1)Selective serotonin reuptake inhibitor, Antidepressant agent, chemically it is S-(+)-5-Isobenzofurancarbonitrile, 1-[3-(dimethyl amino) propyl]-1-(4-fluorophenyl)-1,3-dihydro-oxalate[1]
Limit of Detection and Limit of Quantitation: The limit of detection (LOD) and limit of quantitation (LOQ) were determined for HPLC method
The criteria employed for selecting the mobile phase for the analysis of the drugs were cost involved and time required for the analysis
Summary
Escitalopram oxalate (ESC) is the (Figure 1)Selective serotonin reuptake inhibitor, Antidepressant agent, chemically it is S-(+)-5-Isobenzofurancarbonitrile, 1-[3-(dimethyl amino) propyl]-1-(4-fluorophenyl)-1,3-dihydro-oxalate[1]. Literature survey reveals several spectroscopic [2,3,4], HPLC [5,6] and HPTLC [7,8,9,10,11,12,13,14,15] methods for estimation of Escitalopram oxalate individually as well as combination with other drugs. All the reported HPLC methods used buffer in the mobile phase and long retention time. The present study was aimed to develop a simple, rapid, precise, accurate, and selective chromatographic method for estimation of Escitalopram oxalate in bulk and dosage forms with the use of buffer in the mobile phase in short duration. Chemicals: Bulk sample of ESC was obtained from Plethico Pharmaceuticals Ltd., Indore, India. Preparation of standard stock solutions: Accurately weighed 10.0 mg of ESC was transferred to a 10.0 mL volumetric flask, sufficient amount of methanol was added to dissolve it and volume was made up to IJPC 2 [1] 2012 23-26
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