Abstract
The 3-methyl-2-benzothiazolone hydrazone method has been applied to the determination of erythrocyte membrane sialic acid residues. This method requires a mild oxidation of sialic acid which results in the formation of analogs. Their separation by chromatography, after labeling, allows the choice of the best conditions for this oxidation. The concomitant liberated formaldehyde is determined. This method requires no prior release of sialic acid as opposed to the periodate-thiobarbituric method of Warren (1959, J. Biol. Chem., 234, 1971–1975). These two methods have been compared.
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