Abstract
A new highly sensitive method of microsomal epoxide hydrolase activity has been worked out. The product of styrene oxide enzyme hydrolysis--phenylethyleneglycol (PEG) was extracted by n-butanol, and its aliquot was analysed by high-performance liquid-chromatography on Silica Gel using hexane-isopropanol-water (80:28:2) mobile phase. PEG was detected at 210 nm. The detection limit of PEG was 5 pmol per injection. The coefficient of variation of the method was 3.7%.
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