Abstract
Numerous Plasmodium falciparum isolates from different areas of the world were examined for glucose phosphate isomerase variants by isoelectric focusing and polyacrylamide electrophoresis. The results demonstrate that both methods are required for a comprehensive description and sufficient understanding of glucose phosphate isomerase polymorphism, and isoelectric focusing and polyacrylamide electrophoresis are superior to previously reported electrophoretic techniques for enzymatic analysis of malarial isolates. Conventional acrylamide gel electrophoresis resolved five electrophoretic variants consisting of two phenotypes. Polyacrylamide isoelectric focusing showed three- and six-band electrophoretic patterns. A mixture of three ranges of ampholytes gave optimum resolution. The glucose phosphate isomerase banding patterns showed intraregional variation since both phenotypes were found in the same region. Similar enzyme patterns were found in isolates from different parts of the world.
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