Determination of Enzyme Activity and Vitamin Content of Moringa Oleifera Lam. in Response to Glutathione and Biofertilizer under Water Stress

Abstract

The study was carried out in the canopy belongs to the Horticulture and Landscape Department - College of Agriculture - University of Kerbala in pots with a capacity of 28 kg during the spring growing season 2021 to know the effect of spraying with glutathione, biofertilizer (Algacell) under different water stress levels on the effectiveness of some enzymes and the content of some vitamins of Moringa Oleifera plant. The study was designed as a factorial experiment according to the randomized complete block design (R.C.B.D), as the study included three factors: glutathione with three concentrations (0, 100, 200) mg.L−1 and biofertilizer (Algacell) at two levels (0 and 10) ml. L−1 and water stress with three levels of field capacity (60%, 50%, 40%), and the averages were compared using Dunkin’s multinomial test at a probability level of 0.05. Most of the vegetative growth characteristics were the spraying treatment at a concentration of 200 ml. L−1 achieved the highest rates in terms of (the activity of the enzyme catalase, the activity of the dismutase enzyme, the activity of the peroxidase enzyme, the concentration of ascorbic acid), which reached (2.75%, 28.622%, 6.005 mg.gm−1, 7.561%, 0.377 units.gm−1, 11.777 gm−1, 0.990 gm−1, 275.444 mg.gm−1), respectively, followed by the spray treatment with a concentration of 100 ml. L−1, while the comparison treatment recorded fewer concentrations, while the bio-fertilizer (Algacell) did not achieve a significant difference in all the studied traits, while the water stress treatment excelled at the level of 40% of the field capacity in the traits (the activity of the catalase enzyme, the activity of the dismutase enzyme, the activity of the peroxidase enzyme, the concentration of ascorbic acid, the concentration of α-Tocopherol) which gave respectively (0.446 gm−1, 13.222 gm−1, 1.133 gm−1, 294.167 mg.gm−1, 257.83 μg.gm−1), and the effect of the double and triple interaction between the treatments was similar to their single effects in improving most of the studied traits.