Abstract
A method for the simultaneous determination of robenidine, halofuginone, lasalocid, monensin, nigericin, salinomycin, narasin, and maduramicin residues in eggs by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed. The sample preparation method used a combination of liquid–liquid extraction (LLE) and solid-phase extraction (SPE) technology to extract and purify these target compounds from eggs. The target compounds were separated by gradient elution using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). Tandem mass spectrometry was used to quantitatively and qualitatively analyze the target compounds via electrospray ionization (ESI+) and multiple reaction monitoring mode. The HPLC–MS/MS and UPLC–MS/MS methods were validated according to the requirements defined by the European Union and the Food and Drug Administration. The limits of detection and limits of quantification of the eight coccidiostats in eggs were 0.23–0.52 µg/kg and 0.82–1.73 µg/kg for HPLC–MS/MS, and 0.16-0.42 µg/kg and 0.81-1.25 µg/kg for UPLC–MS/MS, respectively. The eggs were spiked with four concentrations of the eight coccidiostats, and the HPLC–MS/MS and UPLC–MS/MS average recoveries were all higher than 71.69% and 72.26%, respectively. Compared with the HPLC–MS/MS method, utilizing UPLC–MS/MS had the advantages of low reagent consumption, a short detection time, and high recovery and precision. Finally, the HPLC–MS/MS and UPLC–MS/MS methods were successfully applied to detect eight coccidiostats in 40 eggs.
Highlights
Coccidiosis is a highly infectious parasitic disease caused by a single-celled organism of the Eimeriidae species
The eggs were spiked with four concentrations of the eight coccidiostats, and the high-performance liquid chromatography (HPLC)–MS/MS and ultra-performance liquid chromatography (UPLC)–MS/MS average recoveries were all higher than 71.69% and
Compared with the HPLC–MS/MS method, utilizing UPLC–MS/MS had the advantages of low reagent consumption, a short detection time, and high recovery and precision
Summary
Coccidiosis is a highly infectious parasitic disease caused by a single-celled organism of the Eimeriidae species. Coccidiosis is one of the most common diseases that causes serious economic losses to farmers of modern, high-density, intensive poultry farms. To reduce the economic loss caused by coccidiosis, anticoccidial drugs are widely used in the treatment of coccidiosis. Anticoccidial drugs include a wide range of polyether antibiotics (lasalocid, monensin, salinomycin, etc.), synthetic chemical drugs (sulfanilamide groups, halofuginone, robenidine, etc.) and so on. Salinomycin, and lasalocid have good anticoccidial activity and promote growth function [4]. The mechanism of the anticoccidial activity of antibiotics is different from that of synthetic chemical drugs, so cross-resistance does not occur between the classes, and they can be used interchangeably or in combination to prolong and enhance the ability of disease protection
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