Determination of eight biogenic amines in selected seafood products by MSPD extraction followed by UHPLC-Orbitrap MS

Abstract

A quantitative method was previously explored in canned and frozen tuna utilizing matrix solid-phase dispersion for extraction of agmatine, cadaverine, histamine, phenyethylamine, putrescine, tryptamine, tyramine, and urocanic acid with separation and quantification of these compounds by ultra-high performance hydrophilic interaction chromatography (UHPLC-HILIC) with orbitrap mass spectrometric detection. In the current work, the method was extended onto analysis of biogenic amines in non-tuna food matrices known to be associated with scombroid poisoning, including canned/frozen mackerel and frozen mahi–mahi. For method applicability across a wider range of sample types, a non-scombroid species, sockeye salmon, as well as a Thai fish sauce made with anchovy, were also tested. The method was validated with respect to accuracy, repeatability, linearity, and limits of detection and quantitation for all eight analytes in each of these sample types. Recoveries fell in the 80–109% range, percent relative standard deviation ranged from 0.2 to 9.5%, and correlation coefficients for linear regressions were not less than 0.9951 for all samples and analytes. Limits of detection and quantitation were found to be appropriate for the analysis for each sample and analyte combination.