Abstract
A high performance liquid chromatography (HPLC) method for the determination of echinocystic acid in spina gleditsiae was established. Chromatographic conditions were as follows: The separation was performed on Dikma Kromasil C18 column (250 mm × 4.6 mm, 5 μm) with column temperature kept at 30 °C. The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (45:55) at a flow rate of 1.0 mL/min with detection wavelength set at 215 nm. The sample input was 10 μL. The results showed that echinocystic acid had a good linear relationship in the concentration range of 3.16-63.4 μg/mL. The average recovery was 98.21% and RSD was 0.93% (n = 9). The established method was proved to sensitive, accurate, simple, repeatable and applicable for the quality control of the extracts of spina gleditsiae.
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