Abstract
A method is described for the determination of low nanogram concentrations of doxepin, plus its primary active metabolite desmethyldoxepin, in small amounts of serum. The procedure employs a hexane extraction of the biologic specimen after salting out the drug from the biologic matrix with sodium carbonate/sodium bicarbonate powder. Doxepin and desmethyldoxepin are derivatized with 2',2',2',trichloroethylchloroformate to form a single product that is highly sensitive to the electron-capture detector. The recovery of doxepin and desmethylcloxepin from serum after derivatizetion with the polyhalogenated chloroformate reagent is 95 +-2.5%. Quantitation Is based upon the utilization of dothlepin, a compound that has a molecular structure similar to that of doxepln, as the Internal standard.
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