Abstract
Poly (acridine red) modified glassy carbon electrode was used for the detection of dopamine in the presence of ascorbic acid in a pH 7.4 phosphate buffer solutions (PBS) by cyclic voltammetry and differential pulse voltammetry. The major difficulty of the overlapped oxidation potential of ascorbic acid could be overcome through the distinct attractive ability of poly (acridine red) film to cationic dopamine and anionic ascorbic acid. The results showed that the dopamine anodic peak current and the concentration of dopamine had a linear relationship in the range of 1.0×10-7 ~ 1.0×10-4 mol dm-3. The detection limit (S/N=3) obtained by differential pulse voltammetry was 1.0×10-9 mol dm-3. The relative standard deviation of 10 successive scans was 2.07 % for 1.0×10-6 mol dm-3 DA. Ascorbic acid had hardly interference with the determination of dopamine. The proposed method exhibits good recovery and reproducibility.
Highlights
Dopamine (DA) is a kind of neurotransmitter in mammalian central nervous system
From the second cycle on, two obvious anodic peaks (b, c) appeared, larger peaks were observed upon continuous scanning. These facts indicated acridine red was deposited on the surface of glassy carbon electrode (GCE) by electropolymerization mode
A uniform adherent carmine polymer film was formed on the GCE surface during electropolymerization
Summary
Dopamine (DA) is a kind of neurotransmitter in mammalian central nervous system. Extreme abnormalities of DA levels are symptoms of several diseases such as parkinsonism. In order to overcome this problem, double - layer film [6,7,8,9] modified electrodes are used, which are coated first with an electroactive material having catalytic effect on the oxidation of DA and with a Nafion layer. A number of researchers have employed polymeric film modified electrode to detect DA. AA had no obvious response at this modified electrode It is said the existence of AA did not interfere with the determination of DA. Dopamine and ascorbic acid were purchased from ICN Biomedicals Inc (USA) They and all the other chemical reagents are of analytical-reagent grade. A conventional three-electrode system was employed with a bare or poly (acridine red) modified electrode (3.0 mm in diameter) as the working electrode, a saturated calomel electrode (SCE) as the reference electrode, and a platinum electrode as the counter electrode. All potentials reported in this paper were referenced to the SCE
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