Abstract
After extraction overnight of Saccharomyces cerevisiae (baker's yeast) with dilute alkali, to remove nucleotide pools and RNA, acidification gave an insoluble residue from which DNA guanine and adenine were extracted, in the free form, by 1M perchloric acid at room temperature, and determined by cation-exchange chromatography. Percentage DNA in the yeast was computed as 0.062 × μmol/g of guanine + adenine. Preliminary extraction of nucleotides was unnecessary, and gave low results if acid reagents (perchloric or trichloroacetic acids) were used. Several reagents were tested for their ability to extract the nucleotide pool from intact yeast without significant effect on cell RNA content. The content of guanine + adenine in pool-free yeast was taken as giving the sum of RNA + DNA purines, RNA purines being found by difference.
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