Abstract
A high performance liquid chromatographic method was established for the simultaneous quantitative determination of diphenyl (DP), o-phenylphenol (OPP) and thiabendazole (TBZ) in citrus fruits and banana. Dibasic sodium phosphate and anhydrous sodium sulfate were added to homogenized samples. The three food additives were extracted with ethyl acetate. They were separated within 20min on a 15cm reverse phase “Zorbax ODS” column with methanol-0.1N ammonia water as the mobile phase. DP was detected spectrophotometrically at 254nm. OPP and TBZ were detected spectrofluorometrically at an excitation wavelength of 285 or 305nm, with emission at 350nm. Recoveries of DP, OPP and TBZ from orange were 92.2%, 96.4% and 91.4%, respectively.
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